a multi-locus study for detection of cryptosporidium species isolated from calves population, liverpool uk
نویسندگان
چکیده
cryptosporidium is an obligate intracellular protozoan parasite infecting a wide range of hosts. the current study investigated the genetic profile of cryptosporidium species in calves in liverpool, england. fifty-two calve fecal samples were collected from a farm and initially screened by auramine phenol, modified ziehl-neelsen and elisa. pcr analysis of 18s rrna gene was carried out for the positive samples. then, positive pcr samples were genotyped by an 18s rrna- based pcr-rflp, cowp - based pcr- rflp pcr of gp60 and hsp70 genes. additionally, sequence analysis was carried out based on representative isolates of four loci. cryptosporidium oocysts and antigens were detected in 34 out if 52 (65.4%) samples using screening techniques. genotype analysis showed the presence of c. hominis and c. parvum in one and thirteen samples, respectively. furthermore, subtypes of c. hominis ib, c. parvum iia c. parvum subtype 2 were identified by gp60 and hsp70 sequences, respectively. these findings indicate the diversity of the molecular characteristics of cryptosporidium species in calves’ isolates. moreover, referring to the literature we report two new subtypes of c. parvum iia and a rare case of c. hominis ib in calves population.
منابع مشابه
A Multi-locus Study for Detection of Cryptosporidium Species Isolated from Calves Population, Liverpool UK
Cryptosporidium is an obligate intracellular protozoan parasite infecting a wide range of hosts. The current study investigated the genetic profile of Cryptosporidium species in calves in Liverpool, England. Fifty-two calve fecal samples were collected from a farm and initially screened by Auramine phenol, modified Ziehl-Neelsen and ELISA. PCR analysis of 18S rRNA gene was carried out for the p...
متن کاملA Multi-Locus Study for Detection of Cryptosporidium Species Isolated from Calves Population, Liverpool; UK
Cryptosporidium is an obligate intracellular protozoan parasite infecting a wide range of hosts. The current study investigated the genetic profile of Cryptosporidium species in calves in Liverpool, England. Fifty-two calve fecal samples were collected from a farm and initially screened by Auramine Phenol, modified Ziehl-Neelsen and ELISA. PCR analysis of 18S rRNA gene was carried out for the p...
متن کاملa multi-locus study of cryptosporidium parasites isolated from patients living in iran, malawi, nigeria, the united kingdom, and vietnam.
background: cryptosporidium species are important cause of diarrheal diseases in both developing and developed countries. this study aimed to compare the perfor-mance of several molecular methods for identification of cryptosporidium species, and to detect genetic variation among each of these species isolated from iran, ma-lawi, nigeria, vietnam and the united kingdom. methods: the oocysts dna...
متن کاملA Multi-Locus Study of Cryptosporidium Parasites Isolated From Patients Living In Iran, Malawi, Nigeria, the United Kingdom, and Vietnam
BACKGROUND Cryptosporidium species are important cause of diarrheal diseases in both developing and developed countries. This study aimed to compare the performance of several molecular methods for identification of Cryptosporidium species, and to detect genetic variation among each of these species isolated from Iran, Malawi, Nigeria, Vietnam and the United Kingdom. METHODS The oocysts DNA s...
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attempts have been made to study the thermodynamic behavior of 1,3 butadiene purification columns with the aim of retrofitting those columns to more energy efficient separation schemes. 1,3 butadiene is purified in two columns in series through being separated from methyl acetylene and 1,2 butadiene in the first and second column respectively. comparisons have been made among different therm...
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عنوان ژورنال:
international journal of molecular and cellular medicineجلد ۳، شماره ۱، صفحات ۳۵-۴۲
کلمات کلیدی
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